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31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

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FR_CEA-GEN-GM3

On-site

Test
genoscope

Biocatalyst

Genome Mining

Functional Exploration from biodiversity

High throughput enzyme screening

FR_CEA-GEN-CAT

LCAB

Units of access: 95 genes

Quantity offered: 80 middle size projects

Total offered: 7500 genes

About the Platform 

Located at Genoscope (CEA sequencing center for environmental genomics), this platform is dedicated to broaden the field of industrial applications by mining genome by a sequence driven approach to discover novel enzymatic activities. To explore the microbial biodiversity of genes coding for enzymes, we have developed a high throughput process to select, clone the corresponding gene, express and screen the enzymes for the desired activity.

The use of this platform led to the discovery of new enzyme catalysing new nitrilases (Vergne-Vaxelaire et al., 2013), baeyer-villigerases (Reignier et al., 2014), dioxygenases (Baud et al., 2014), aldolases (Helaine et al., 2015; de Berardinis et al., 2016; Victor et al., 2018), aminotransferases (Heuson et al., 2016; Guerard-Helaine et al., 2017, Heuson et al., 2018), wild type amine deshydrogenases (Mayol et al., 2016), sugar kinases (Vergne- Vaxelaire et al., 2018).

TNA Map France

T.N.A. Offer

- From a set of experimentally known enzymes provided by the user, representative sequences from biodiversity will be selected using our bioinformatic tool

- The corresponding genes will be cloned in a expression vector with/without a Histag depending the user need

- The gene collection will be over-expressed in E. coli and the cell-free extract will be prepared in 96-wells

- The screening on 5 substrates maximum will be performed using UV spectrophotometry or Mass-spectrometry (LC/MS) detection. Nevertheless, expensive substrates (>100 euros/mg) will be provided by the user

- At the end of the process a list of enzymes catalysing the desired reaction will be delivered to the user

- Further work can then be considered on a collaboration basis

More Info

Genomic resources:

- metagenome from a wastewater treatment plant

-1100 prokaryote strains (including archaea) from 580 genus

Screening by:

- spectrophotometry or Mass-spectrometry (LC/MS)

Today Genoscope collections:

~11000 genes
Enzymes families: transaminases, aldolases, baeyer-villigerases, esterases, nitrilases, transketolases, levanases, sugar kinases 

CEA

The French Alternative Energies and Atomic Energy Commission (CEA) is dedicated to connecting the worlds of research and industry. It is one of the most prominent players in research and innovation in France and throughout the world. It is a key player in research, development and innovation in four main areas: defence and security, nuclear energy (fission and fusion), technological research for industry, fundamental research in the physical sciences and life sciences