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24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

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TNA Results

TNA Results

1st Open Call | Selected Projects

Optimisation of production of flavin-containing oxidoreductases for industrial application

Plant-based bio-encapsulation strategies for oral veterinary vaccines

High-resolutions fluxomics of industrial cyanobacteria

Integrative and comparative analyses of response of Pichia pastoris reaction network to expression systems designed with engineered promoters

Promoters4Pichia | 2 INSTALLATIONS
Innovative methanol free promoter comparison for Pichia pastoris for scalable bioprocesses

2nd Open Call | Selected Projects

SporeDel | Italy | 2 INSTALLATIONS
Optimisation of the Spore-Display system for the mucosal delivery of drugs and antigens

Yeast Glicerol | Germany | 2 INSTALLATIONS
Reconstruction and validation of a metabolic model for the yeast Saccharomyces  cerevisiae growing on glycerol as the sole carbon source

Galambr | Poland | 1 INSTALLATION
Hydrolisis of pectic oligosaccharides from sugar beet pulp to galacturonic acid in an enzyme membrane reactor

Novel (ligno)cellulose degrading enzyme for expression in the  thermoacidofilic Archaeon Sulfolobus acidocaldarius

3rd Open Call | Selected Projects

Evocolour | Netherlands | 2 INSTALLATIONS
The evolution and genetics of Flavobacteria forming structurally coloured colonies for sustainable biomaterials

Structural colour is widely found in nature and is defined by at least partially ordered nanostructures which interact with light to create vivid, angle-dependent colour. Structural colour (SC) is quite distinct from chemical pigments and offers superior properties (intensity, resistance to bleaching, advanced optical effects) compared to pigments and dyes. SC is familiar to us, for example in the way the feathers of the peacock catch the light. SC was observed by Hook and Newten in the 17th century and thanks to the efforts of optical physicists the study of SC at the level of optics is advanced. Despite the widespread distribution of SC in life (insects, arachnids, cephalopds, plants, birds) the genetics and genomics of SC in life is poorly studied and so there is no genomics view. However, we have developed the bacterium IR1, which aligns cells in colonies to form vivid SC, and used transposon mutagenesis to identify some of the genes involved (Johansen et al, 2018).

The aim of this project is to develop IR1 as a model organism with the modification of SC using synthetic genes (modified genes from IR1 or transgenics by importing genes involved in colour processes from other organisms). The aim is both to understand SC and create new 'production strains' which may be used to create sustainable biomaterials. This is already possible on a small scale, the work in this project is likely to make such materials more feasible and so support a disruptive innovation to reduce the use of dyes which often have a high carbon footprint and create problems in pollution (eg textiles). The second aspect of the project is long term automated cultivation to establish rapidly growing and stable production strains and understand the evolution of colour.

This project is designed to showcase the power of synthetic biology in rapidly developing a new a area of strain engineering for a form of colour previously not exploited.

Extreme | Tunisia | 1 INSTALLATION
Genomic approach for extremozymes screening of thermophilic/anaerobic Caldicoprobacter algerensis strain

In this project we have, as general objective, the screening of few extremozymes from the thermophilic/anaerobic Caldicoprobacter algerensis strain [1] using genomic approach.  The major specific goal of this project is the determination of the whole genome sequence of this strain followed by sequence assembly and annotation. We will then exploit the obtained sequence to identify potential ORFs encoding extremozymes with industrial interest. A particular interest will be done for CAZymes (Xylanase, beta-glucanase, Mannanase etc) useful for lignocellulose degradation and for animal feed, and for isomerases useful for the natural sweet sugar (Tagtaose, Fructose, Palatinose) production.

HMOzymes | Spain | 1 INSTALLATION
Screening of mutant libraries to identify the best biocatalysts for biotechnological production of human milk oligosaccharides

Infant formula milk tries to mimic human milk as closely as possible, since it is well-known that breast milk has considerable implications in the development of the infant intestinal microbiota in the first years of life. Human milk is considered to be unique in terms of its content of complex oligosaccharides. They are abundant in human milk and their role in the development of intestinal flora blocking the attachment of pathogens and modulating immune system of the infant make them of great interest. To date, 150 chemical structures of human milk oligosaccharides (HMO) had been identified. Two tetrasaccharides are the core structures: the lacto-N-tetraose (type 1) characterized by lacto-N-biose (Galbeta1,3GlcNAc) and lactose units, and lacto-neo-N-tetraose (type 2) formed by N-acetyllactosamine (Galbeta1,4GlcNAc) and lactose. They are elongated to decaose and then, decorated with fucosyl and sialic residues. Humans are the only species in which type 1 HMO dominate over type 2. Until now, large scale synthesis of type 1 HMO has not been possible by any synthetic methodology.

With our expertise on engineering CAZYmes, now we are eager to reach efficient synthesis of the type 1 HMO. For this, we focus on the GH20 family hexosaminidases where lacto-N-biosidases enzymes are present in the gut microbiota of breast-fed infants and able to hydrolyse the type 1 HMO core structures. We have defined the minimal functional structure of Bifidobacterium bifidum lacto-N-biosidase and identified the hot-spots positions on the sugar binding sites using rational design and in silico methods to modulate synthase/hydrolase activities ratio and promote transglycosylation. In this framework, this project aims at obtaining lacto-N-biosidase variants library using random mutagenesis to identify other positions (hot spots) of the catalytic site not discovered by rational design. Then this library will be screened to analyse the mutants with the best transglycosylation/hydrolysis ratio.

Pilot scale process development for microbial proteins

Calidris Bio aims to bring a technology to the market to produce microbial protein for feed and food purposes. Calidris Bio is developing a production process consisting of fermentation and conditioning of the microbial biomass. The fermentation process was optimised at lab-scale (2L fed-batch and continuous fermentation) with a productivity of 10g/L/h. The next step is to scale-up the process to pilot scale. A concomitant life cycle analysis will be performed to pinpoint hotspots thus improving the environmental footprint of the process. The novelty of the process consists of steering the biomass composition and metabolic state by adjusting substrate composition and feeding rate; working in a continuous process mode at large scale (>100L); steering the biomass composition when running in continuous mode.

Upscaling and optimisation of microalgae production for bivalves hatchery

Farming of shellfish and seaweed is still new in Denmark and on a commercial scale is primarily limited to the production of longline mussels. It is mainly for mussels, that aquaculture production rely on natural spatfall. For all other species, such as oysters and macroalgae, hatcheries for the production of spat or seeding material are necessary. A major bottleneck in all bivalve hatchery is the food production, both in term of quantity and quality.

The native European flat oysters, is a key species in Denmark, both from its high commercial value, but also for restoration of reef, providing ecosystem good and services. A large R&D effort has been carried out at the Danish Shellfish Centre (DSC) to successfully develop a reliable production.

The next step is to scale up the production, which requires larger and better facilities. A new hatchery at DSC is nowadays under development and will be built during the year 2020 and finished in 2021. In this new facility a large part of the area is devoted to the production of microalgae to ensure the various food needs of the different bivalves produced.

One of the main development in this area is the use of photo-bioreactor and raceway ponds that can ease the production and help upscaling the level of production in the hatchery.

Although photo-bioreactors are well functioning for some microalgae species, the challenge is now to adapt them for our needs.

In order to develop and implement new microalgae production technologies at the future facility we contacted various companies and Universities. AlgoSolis in France seems the logical place to learn and collaborate on microalgae production, in view of their work at their R&D platform. Discussions lead to the opportunity of an internship application at AlgoSolis for exchange and training for real scale production using various technologies.