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Dernière mise à jour : Mai 2018

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PlatBioEng-SysBiol

Systems metabolic Engineering | Bioreactor-Scale Strain Characterisation | Multilevel Omics Analyses | Strain engineering | Genome Scale Modelling

PlatBioEng-SysBiol

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About the Platform 

The Systems Biology Lab of the UAB is focused on the systems-level engineering of microbial cell factories for production of recombinant proteins and high value biochemicals. The lab has a wide experience in strain engineering and metabolic/physiological characterisation under bioprocess-like conditions, ensuring a continuum with bioprocess development and optimisation. Specifically, our infrastructure allows to carry out different steps of the strain development process, including cell metabolic modelling, strain design and construction, as well as metabolic/physiological characterisation under bioprocess-relevant testing conditions, i.e. at different bioreactor scales & operational modes. Such metabolic phenotyping studies can include multilevel omics analyses (transcriptomics, proteomics, metabolomics, 13C-MFA) assisted by genome scale modelling.

 

Offer

The SysBioUAB infrastructure offers an access of 3 months to the facilities and equipment, which can be adapted to a wide range of applications:

  • In-silico projects focused on cell metabolic modelling and analysis including genome scale modelling and raw data treatment for analysis and interpretation
  • Rational strain design and construction
  • Metabolic/physiological characterisation of engineered strains in bioreactor-scale cultivations (different scales and operational modes)
  • For these tests, quantitative physiological analyses can be carried out, including biomass compositional analyses, 13C-labelling experiments, as well as multilevel omics analyses supported by genome scale modelling.

More Info

The SysBioUAB Lab offers a wide range of equipment:

  • Molecular Biology lab for strain construction: incubators, electroporator, thermocyclers, etc.
  • Fully equipped lab scale bioreactors for strain characterisation: on-line and off-line data acquisition devices for quantitative physiological studies
  • Automated fast sampling device for metabolomics and 13C-labelling experiments at bioreactor scale
  • Hardware and software for physiological raw data analysis, multiscale whole-cell metabolic reconstruction, modelling and analysis (COBRA toolbox, 13C-MFA, etc.)

Units of access: 3 Months

  • Quantity offered: 1
  • Total offered: 3 months
  • Type of access: on-site

SERVICE INSTALLATION CODE: ES_UAB-PlatBioEng-SysBiol

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The Universitat Autònoma de Barcelona (UAB) is one of the major public universities in Spain and coordinates the scientific and technological UAB-CIE Sphere, which comprises science and technology services, research centres, institutes and university hospitals. Within the UAB, the Department of Chemical, Biological and Environmental Engineering (DEQBA) is a reference of Southern Europe in the fields of Biological Engineering and Environmental Technologies.

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