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PlatBioEng-BioEng

Bioprocess Engineering | Continuous and Fed-Batch Bioreactor Cultures/Yeast/ Macrokinetic and Physiological Studies | Process Development and Optimisation/Process Monitoring and Control

PlatBioEng-BioEng

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About the Platform 

The UAB's Bioprocess Engineering Lab is a facility that hosts R&D+I projects in the field of microbial cultivation process development, with strong emphasis in bioprocess optimization, including cultivation strategies in different operational modes (batch, continuous, fed-batch), bioprocess on line monitoring, modelling and control, as well as DoE for process scale-up. Notably, the Lab has a wealth of experience in high cell density cultivation of the methylotrophic yeast Pichia pastoris. The BioEng Lab also supports strain metabolic/physiological characterisation studies under bioprocess-like conditions performed by the SysBiol UAB Lab.

Offer

Integrated development and intensification of cultivation processes for the production of biochemicals and recombinant proteins using different microbial cell factories, with emphasis in yeast and bacteria.

Strain testing at laboratory bioreactor scale: Macrokinetic and physiological characterisation of new strains and genetic parts (e.g. promoters) under bioprocess-like conditions: Determination of key parameters for process development and optimisation using continuous cultures (supported by targeted transcriptomics) for selection of optimal fed-batch cultivation strategies and parameters.

Cultivation process optimisation (medium composition, process mode, feeding strategies), as well as selection of process parameters for scale-up and transfer of lab scale cultivation processes to pilot plant. Novel fed-batch process intensification strategies driven by on-line monitoring of key fermentation parameters.

More Info

The facility, which has certification/permit to work with GMOs, offers a wide range of equipment:

  • HPLC, GC, GC-MS, HPLC-MS, FPLC
  • Cell disruptor, centrifuges, lyophilizer
  • Fluorimeter, flow cytometer, spectrophotomer
  • Bioreactors up to 5 L equiped with on-line gas (CO2, O2) and methanol methanol analyzers

Units of access: 3 Months

  • Quantity offered: 1
  • Total offered: 3 months
  • Type of access: on-site

SERVICE INSTALLATION CODE: ES_UAB-PlatBioEng-BioEng

UAB logo1

The Universitat Autònoma de Barcelona (UAB) is one of the major public universities in Spain and coordinates the scientific and technological UAB-CIE Sphere, which comprises science and technology services, research centres, institutes and university hospitals. Within the UAB, the Department of Chemical, Biological and Environmental Engineering (DEQBA) is a reference of Southern Europe in the fields of Biological Engineering and Environmental Technologies.

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